Lab tests show risks of using CRISPR gene editing on embryos

A lab experiment aimed at fixing defective DNA in human embryos shows what can go wrong with this type of gene editing and why leading scientists say it’s too unsafe to try. In more than half of the cases, the editing caused unintended changes, such as loss of an entire chromosome or big chunks of it.

Columbia University researchers describe their work Thursday in the journal Cell. They used CRISPR-cas9, the same chemical tool that a Chinese scientist used on embryos in 2018 to help make the world’s first gene-edited babies, which landed him in prison and drew international scorn.

The tool lets scientists cut DNA in a precise spot and has profound potential for good — it’s already used to raise better crops and livestock, holds promise for treating diseases and earned its discoverers a Nobel Prize earlier this month.

But using it on embryos, sperm or eggs makes changes that can pass to future generations. Several international panels of scientists and ethicists have said it’s too soon to know whether that can be done safely, and the new Columbia work shows the possible harm.

“If our results had been known two years ago, I doubt that anyone would have gone ahead” and tried it on embryos intended for pregnancy, said biologist Dieter Egli, who led the study.

Researchers made 40 embryos with eggs from healthy donors and sperm from a man with a gene mutation -- a single letter missing in the DNA alphabet -- that causes blindness. Editing was aimed at adding the missing letter so the gene would work.

In some embryos, the editing was tried at fertilization, thought to be the best time for such attempts. Other embryos were edited when they contained two cells and were almost two days old. Cells then were analyzed at various stages of development to see how many...